The effect of layer-by-layer chitosan-hyaluronic acid coating on graft-to-bone healing of a poly(ethylene terephthalate) artificial ligament

J Biomater Sci Polym Ed. 2012;23(1-4):425-38. doi: 10.1163/092050610X551989. Epub 2011 Jan 21.


Surface coating with an organic layer-by-layer self-assembled template of chitosan and hyaluronic acid on a poly(ethylene terephthalate) (PET) artificial ligament was designed for the promotion and enhancement of graft-to-bone healing after artificial ligament implantation in a bone tunnel. The results of in vitro culturing of MC3T3-E1 mouse osteoblastic cells supported the hypothesis that the layer-by-layer coating of chitosan and hyaluronic acid could promote the cell compatibility of grafts and could promote osteoblast proliferation. A rabbit extra-articular tendon-to-bone healing model was used to evaluate the effect of this kind of surface-modified stainless artificial ligament in vivo. The final results proved that this organic compound coating could significantly promote and enhance new bone formation at the graft-bone interface histologically and, correspondingly, the experimental group with coating had significantly higher biomechanical properties compared with controls at 8 weeks (P < 0.05).

MeSH terms

  • 3T3 Cells
  • Animals
  • Bone Transplantation / adverse effects*
  • Bone and Bones / drug effects
  • Bone and Bones / pathology
  • Bone and Bones / surgery*
  • Cell Proliferation / drug effects
  • Chitosan / chemistry*
  • Coated Materials, Biocompatible / chemistry
  • Coated Materials, Biocompatible / pharmacology*
  • Hyaluronic Acid / chemistry*
  • Ligaments / drug effects*
  • Ligaments / pathology
  • Male
  • Mechanical Phenomena
  • Mice
  • Polyethylene Terephthalates / chemistry*
  • Rabbits
  • Surface Properties
  • Wound Healing / drug effects


  • Coated Materials, Biocompatible
  • Polyethylene Terephthalates
  • Hyaluronic Acid
  • Chitosan