A photopolymerized antimicrobial hydrogel coating derived from epsilon-poly-L-lysine

Biomaterials. 2011 Apr;32(11):2704-12. doi: 10.1016/j.biomaterials.2010.12.040. Epub 2011 Jan 22.


Hydrogels made from epsilon-poly-l-lysine-graft-methacrylamide (EPL-MA) have been found to have impressive wide spectrum antimicrobial activity against both bacteria (specifically Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens and Staphylococcus aureus) and fungi (specifically Candida albicans and Fusarium solani). The EPL-MA hydrogel also possesses in vitro biocompatibility and EPL-MA solution is relatively non-hemolytic: the concentration needed for onset of human red blood cell (hRBC) hemolysis is 12,500 μg/mL so that the selectivity for the pathogenic microorganisms over hRBCs is 230-1560. Further, EPL-MA hydrogel can be conveniently ultraviolet-immobilized onto plasma-treated plastic surfaces to form thin highly adherent antimicrobial hydrogel coatings for medical devices and implants.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Infective Agents / chemistry*
  • Anti-Infective Agents / pharmacology*
  • Bacteria / drug effects*
  • Candida albicans / drug effects
  • Escherichia coli / drug effects
  • Fungi / drug effects*
  • Hemolysis / drug effects
  • Humans
  • Hydrogel, Polyethylene Glycol Dimethacrylate / adverse effects
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry*
  • Microbial Sensitivity Tests
  • Polylysine / chemistry*
  • Pseudomonas aeruginosa / drug effects
  • Serratia marcescens / drug effects
  • Staphylococcus aureus / drug effects


  • Anti-Infective Agents
  • Polylysine
  • Hydrogel, Polyethylene Glycol Dimethacrylate