Abstract
A method was developed for detection of Listeria monocytogenes by polymerase chain reaction amplification followed by agarose gel electrophoresis or dot blot analysis with a 32P-labeled internal probe. The technique identified 95 of 95 L. monocytogenes strains, 0 of 12 Listeria strains of other species, and 0 of 12 non-Listeria strains.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria / isolation & purification
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Evaluation Studies as Topic
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Humans
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Listeria / isolation & purification
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Listeria monocytogenes / genetics
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Listeria monocytogenes / isolation & purification*
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Listeria monocytogenes / pathogenicity
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Polymerase Chain Reaction / methods*
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Polymerase Chain Reaction / statistics & numerical data
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Sensitivity and Specificity
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Species Specificity