8-Hydroxy-2'-deoxyguanosine is a prognostic mediator for cardiac event

Eur J Clin Invest. 2011 Jul;41(7):759-66. doi: 10.1111/j.1365-2362.2010.02465.x. Epub 2011 Jan 25.

Abstract

Background: DNA in the nucleus is one of the major targets of reactive oxygen species (ROS), and oxidative DNA damage has been implicated in the pathogenesis of chronic heart failure. 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is produced from deoxyguanosine in DNA by ROS. The purpose of this present study was to examine the clinical significance of serum 8-OHdG levels in patients with heart failure.

Methods: We measured serum 8-OHdG levels in 230 patients with chronic heart failure and 42 control subjects without heart failure by sandwich enzyme-linked immunosorbent assay. Patients were prospectively followed during a median follow-up period of 472 days with the end points of cardiac death or progressive heart failure requiring re-hospitalization.

Results: Serum 8-OHdG concentrations were higher in patients with heart failure than in control subjects (P < 0·001) and increased with advancing New York Heart Association (NYHA) functional class (P < 0·001). Normal upper limit of 8-OHdG level was determined as mean ± 2SD value from 42 control subjects (0·40 ng mL(-1)). Abnormally high serum 8-OHdG levels (> 0·40 ng mL(-1)) were observed in 21·2%, 43·1%, 42·6% and 69·4% through NYHA I to IV (P < 0·001). A total of 66 cardiac events occurred during a follow-up period, and Kaplan-Meier survival curves demonstrated that cardiac event rate was markedly higher in patients with high 8-OHdG levels than in those with normal 8-OHdG levels (62·4% vs. 29·6%, P = 0·0007).

Conclusions: Serum 8-OHdG levels provide important prognostic information for the risk stratification of patients with heart failure.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aged
  • Aged, 80 and over
  • Biomarkers / blood
  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / blood
  • Disease Progression
  • Epidemiologic Methods
  • Female
  • Heart Failure / blood*
  • Heart Failure / diagnostic imaging
  • Heart Failure / etiology
  • Humans
  • Male
  • Middle Aged
  • Prognosis
  • Ultrasonography

Substances

  • Biomarkers
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine