Flavonol-rich Fractions of Yaupon Holly Leaves (Ilex Vomitoria, Aquifoliaceae) Induce microRNA-146a and Have Anti-Inflammatory and Chemopreventive Effects in Intestinal Myofibroblast CCD-18Co Cells

Fitoterapia. 2011 Jun;82(4):557-69. doi: 10.1016/j.fitote.2011.01.013. Epub 2011 Jan 22.

Abstract

Polyphenolics extracted from yaupon holly (Ilex vomitoria, Aquifoliaceae) (YH) leaves were investigated in human colon cells for their chemopreventive and anti-inflammatory activities. An activity-guided fractionation allowed the selection of YH flavonol-rich fraction due to its preferential inhibition of HT-29 colon cancer viability over the normal CCD-18Co colon cells. Quercetin and kaempferol 3-rutinosides, main components identified in this fraction, protected CCD-18Co cells against reactive oxidative species (ROS) in part due to increased activity of antioxidant enzymes. In addition, up-regulation of microRNA-146a (miR-146a) known as a negative regulator of pro-inflammatory NF-κB activation was the underlying molecular mechanism that protected CCD-18Co from inflammation.

MeSH terms

  • Anti-Inflammatory Agents / analysis*
  • Antineoplastic Agents, Phytogenic / analysis*
  • Antioxidants / analysis
  • Biotransformation / drug effects
  • Cell Survival
  • Drug Evaluation, Preclinical
  • Flavonoids / analysis*
  • HT29 Cells
  • Humans
  • Ilex vomitoria / chemistry*
  • Lipopolysaccharides
  • MicroRNAs / metabolism*
  • Phenols / analysis*
  • Plant Extracts / pharmacology
  • Polyphenols
  • Transcription Factors / metabolism

Substances

  • Anti-Inflammatory Agents
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Flavonoids
  • Lipopolysaccharides
  • MIRN146 microRNA, human
  • MicroRNAs
  • Phenols
  • Plant Extracts
  • Polyphenols
  • Transcription Factors