Grape seed proanthocyanidin extract protects human lens epithelial cells from oxidative stress via reducing NF-кB and MAPK protein expression

Mol Vis. 2011 Jan 20:17:210-7.

Abstract

Purpose: Oxidative damage induced by H₂O₂ treatment can irreversibly damage the lens epithelium, resulting in cell death and cataract. Grape seed extract (GSE) is a widely consumed dietary supplement that has the capability to scavenge oxidants and free radicals. GSE contain 70%-95% standardized proanthocyanidins. The study described herein investigated the protective effect of Grape seed proanthocyanidin extract (GSPE) on H₂O₂-induced oxidative stress in human lens epithelial B-3 (HLEB-3) cells and the possible molecular mechanism involved.

Methods: HLE-B3 cells exposed to different doses of H₂O₂ were cultured with various concentrations of GSPE and subsequently monitored for cell viability by the 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) assay. The apoptosis rate and ROS generation were detected by flow cytometric analysis. Expression of NF-кB/P65 and mitogen activated protein kinase (MAPK) proteins were measured by western blot.

Results: GSPE clearly reduced H₂O₂ induced cell apoptosis and reactive oxygen species (ROS) generation and protected HLEB-3 cells from H₂O₂ induced oxidative damage. GSPE depressed H₂O₂-induced activation and translocation of NF-кB/p65. GSPE also depressed H₂O₂-induced phosphorylation of the p38 and c-Jun N-terminal kinase (JNK) proteins of the MAPK family at various time points studied.

Conclusions: GSPE could be useful in attenuation of H₂O₂-induced oxidative stress and the activation of NF-кB and MAPK signaling in HLE-B3 cells, which suggests that GSPE has a potential protective effect against cataractogenesis.

MeSH terms

  • Cell Line
  • Epithelial Cells / drug effects*
  • Flow Cytometry / methods
  • Free Radicals
  • Gene Expression Regulation, Enzymologic*
  • Grape Seed Extract / metabolism*
  • Humans
  • Hydrogen Peroxide / chemistry
  • JNK Mitogen-Activated Protein Kinases / metabolism
  • Lens, Crystalline / drug effects*
  • MAP Kinase Signaling System
  • NF-kappa B / metabolism
  • Oxidative Stress / drug effects
  • Phosphorylation
  • Proanthocyanidins / pharmacology*
  • Reactive Oxygen Species

Substances

  • Free Radicals
  • Grape Seed Extract
  • NF-kappa B
  • Proanthocyanidins
  • Reactive Oxygen Species
  • proanthocyanidin
  • Hydrogen Peroxide
  • JNK Mitogen-Activated Protein Kinases