The function and mechanism of COX-2 in angiogenesis of gastric cancer cells

J Exp Clin Cancer Res. 2011 Jan 25;30(1):13. doi: 10.1186/1756-9966-30-13.


Background: Here we aimed to investigate the effect of COX-2 siRNA on proliferation and angiogenesis of gastric cancer cells.

Methods: The gastric cancer cell line SGC7901 was transfected with COX-2 siRNA, then the growth and angiogenesis of cells were detected by in vitro and in vivo assay. Human microarray, RT-PCR and western blot were used to identify differentially expressed angiogenesis-related molecules in cells with decreased expression of COX-2.

Results: Down-regulation of COX-2 could significantly inhibit the in vitro and in vivo growth of gastric cancer cells, and suppress the migration and tube formation of human umbilical vein endothelial cells. Totally 23 angiogenesis-related molecules were found involved in COX-2-induced angiogenesis suppression. The results of RT-PCR and western blot showed that down-regulation of COX-2 might inhibit VEGF, Flt-1, Flk-1/KDR, angiopoietin-1, tie-2, MMP2 and OPN.

Conclusions: COX-2 might mediate tumor angiogenesis and growth, and could be considered as a target for gastric cancer therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Cell Movement / drug effects
  • Cell Proliferation
  • Cells, Cultured
  • Cyclooxygenase 2 / genetics
  • Cyclooxygenase 2 / physiology*
  • Endothelial Cells / physiology
  • Female
  • Gene Expression Profiling
  • Humans
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Neoplasms, Experimental / blood supply
  • Neoplasms, Experimental / genetics
  • Neovascularization, Pathologic*
  • RNA Interference
  • RNA, Small Interfering / genetics
  • Stomach Neoplasms
  • Umbilical Veins / cytology


  • RNA, Small Interfering
  • Cyclooxygenase 2