Abstract
RNA processing is vital for the high fidelity and diversity of eukaryotic transcriptomes and the encoded proteomes. However, control of RNA processing is not fully established. Σ RNA is a class of conserved large non-coding RNAs (murine Hepcarcin; human MALAT-1) up-regulated in carcinomas. Using antisense technology, we identified that RNA post-transcriptional modification is the most significant global function of Σ RNA. Specifically, processing of the pre-mRNAs of genes including Tissue Factor and Endoglin was altered by hydrolysis of Σ RNA/MALAT-1. These results support the hypothesis that Σ RNA/MALAT-1 is a regulatory molecule exerting roles in RNA post-transcriptional modification.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
MeSH terms
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Alternative Splicing
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Animals
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Antigens, CD / genetics
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Antigens, CD / metabolism
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Carcinoma / metabolism*
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Cells, Cultured
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Endoglin
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Gene Expression Profiling
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Gene Expression Regulation, Neoplastic
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HeLa Cells
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Humans
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Nuclear Proteins / metabolism
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Oligonucleotide Array Sequence Analysis
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Oligonucleotides, Antisense
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RNA Precursors / metabolism
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RNA Processing, Post-Transcriptional*
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RNA, Long Noncoding
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RNA, Neoplasm / metabolism*
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RNA, Untranslated / antagonists & inhibitors
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RNA, Untranslated / genetics
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RNA, Untranslated / metabolism*
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RNA-Binding Proteins / metabolism
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Rats
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Receptors, Cell Surface / genetics
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Receptors, Cell Surface / metabolism
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Serine-Arginine Splicing Factors
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Thromboplastin / genetics
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Thromboplastin / metabolism
Substances
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Antigens, CD
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ENG protein, human
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Endoglin
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MALAT1 long non-coding RNA, human
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Malat1 long non-coding RNA, mouse
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Nuclear Proteins
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Oligonucleotides, Antisense
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RNA Precursors
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RNA, Long Noncoding
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RNA, Neoplasm
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RNA, Untranslated
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RNA-Binding Proteins
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Receptors, Cell Surface
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Serine-Arginine Splicing Factors
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Thromboplastin