Impact of heat and pollution on oxidative stress and CC16 secretion after 8 km run

Eur J Appl Physiol. 2011 Sep;111(9):2089-97. doi: 10.1007/s00421-011-1839-x. Epub 2011 Jan 26.


To investigate the acute effect of a hot, humid and ozone-polluted (O(3)) environment on lung inflammation and oxidative tress of runners performing 8 km time trial run. Using a single-blinded randomized design, 10 male athletes (mean[Formula: see text]= 64.4 mlO(2) kg(-1) min(-1), SD = 4.4) took part in a time trial run in four different environmental conditions: 20°C + 50% relative humidity (rh) (Control); 20°C + 50% rh + 0.10 ppm O(3) (Control + O(3)); 31°C + 70% rh (Heat); 31°C + 70% rh + 0.10 ppm O(3) (Heat + O(3)). Blood samples and nasal lavage were collected post-exercise and analyzed for inflammatory, epithelial damage and oxidative stress markers. Data were analyzed using repeated measures ANOVA with Tukey's post hoc test. A significant increase in CC16 concentration (P < 0.05) and GSH/protein concentration (P < 0.05) in the upper respiratory airways was observed following the 8 km run in the Heat + O(3) trial compared with the control trial. There were no differences in the neutrophil counts between trials. No differences were observed for the other antioxidants analyzed. A hot, humid and ozone-polluted environment (0.1 ppm) elicits an early epithelial damage and antioxidant protection process in the upper respiratory airways of athletes immediately after performing 8 km time trial run.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Air Pollution* / adverse effects
  • Athletes
  • Cross-Over Studies
  • Exercise Test / methods
  • Hot Temperature* / adverse effects
  • Humans
  • Humidity / adverse effects
  • Male
  • Oxidative Stress / physiology*
  • Physical Exertion / physiology
  • Running / physiology*
  • Single-Blind Method
  • Uteroglobin / blood
  • Uteroglobin / metabolism*
  • Young Adult


  • SCGB1A1 protein, human
  • Uteroglobin