Assessment of impact of peptide nucleic acid fluorescence in situ hybridization for rapid identification of coagulase-negative staphylococci in the absence of antimicrobial stewardship intervention

J Clin Microbiol. 2011 Apr;49(4):1581-2. doi: 10.1128/JCM.02461-10. Epub 2011 Jan 26.

Abstract

Peptide nucleic acid fluorescence in situ hybridization (PNA FISH) was instituted at Boston Medical Center for the rapid identification of coagulase-negative staphylococci (CoNS). Without active notification or antimicrobial stewardship intervention, a pre- and postimpact analysis showed no benefit of this assay with respect to the length of hospital stay or vancomycin use.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Academic Medical Centers
  • Anti-Bacterial Agents / therapeutic use*
  • Bacteriological Techniques / methods*
  • Boston
  • Coagulase / metabolism
  • Drug Utilization / statistics & numerical data
  • Female
  • Fluorescence
  • Humans
  • In Situ Hybridization / methods*
  • Length of Stay
  • Male
  • Peptide Nucleic Acids*
  • Staphylococcal Infections / diagnosis*
  • Staphylococcal Infections / microbiology
  • Staphylococcus / enzymology
  • Staphylococcus / genetics
  • Staphylococcus / isolation & purification*
  • Treatment Outcome
  • Vancomycin / therapeutic use

Substances

  • Anti-Bacterial Agents
  • Coagulase
  • Peptide Nucleic Acids
  • Vancomycin