Pyrrolidinone diterpenoid from Isodon excisus and inhibition of nitric oxide production in lipopolysaccharide-induced macrophage RAW264.7 cells

Bioorg Med Chem Lett. 2011 Feb 15;21(4):1279-81. doi: 10.1016/j.bmcl.2010.11.095. Epub 2010 Nov 25.

Abstract

A new pyrrolidinone diterpenoid, excisusin F (1), was isolated from the aerial parts of Isodon excisus (Lamiaceae), together with four known compounds, and their structures were determined mainly by NMR (1D and 2D) and mass spectrometry. Excisusin F (1) and inflexarabdonin E (3) showed potent inhibitory effects of LPS-induced nitric oxide production in RAW264.7 cells with the IC(50) value of 10.4 and 3.8 μM, respectively.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line, Tumor
  • Diterpenes / chemistry*
  • Diterpenes / isolation & purification
  • Diterpenes / pharmacology
  • Isodon / chemistry*
  • Lipopolysaccharides / toxicity
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Magnetic Resonance Spectroscopy
  • Mice
  • Molecular Conformation
  • Nitric Oxide / metabolism*
  • Plant Components, Aerial / chemistry
  • Pyrrolidinones / chemistry*
  • Pyrrolidinones / isolation & purification
  • Pyrrolidinones / pharmacology

Substances

  • Diterpenes
  • Lipopolysaccharides
  • Pyrrolidinones
  • excisusin F
  • inflexarabdonin E
  • Nitric Oxide