Circulating cell-derived microparticles in women with pregnancy loss

Am J Reprod Immunol. 2011 Sep;66(3):199-208. doi: 10.1111/j.1600-0897.2010.00972.x. Epub 2011 Jan 31.


Problem: To analyze cell-derived microparticles (cMP) in pregnancy loss (PL), both recurrent miscarriages (RM) and unexplained fetal loss (UFL).

Method of study: Non-matched case-control study was performed at Vall d'Hebron Hospital. Cell-derived microparticles of 53 PL cases, 30 with RM, 16 with UFL, and 7 (RM + UFL), were compared to 38 healthy pregnant women. Twenty healthy non-pregnant women act as controls. Cell-derived microparticles were analyzed through flow cytometry. Results are given as total annexin (A5+), endothelial-(CD144+/CD31+ CD41-), platelet-(CD41+), leukocyte-(CD45+) and CD41- c-MP/μL of plasma. Antiphospholipid antibodies (aPLA) were analyzed according to established methods.

Results: Comparing PL versus healthy pregnant, we observed a significant endothelial cMP decrease in PL. When comparing RM subgroup with controls, we observed significant decreases in endothelial cMP. When comparing the PL positive for aPLA versus PL-aPLA-negative, no cMP numbering differences were seen.

Conclusion: Pregnancy loss seems to be related to endothelial cell activation and/or consumption. A relationship between aPLA and cMP could not be demonstrated.

MeSH terms

  • Abortion, Habitual / blood*
  • Adult
  • Annexins / blood
  • Antibodies, Antiphospholipid / blood
  • Antigens, CD / blood
  • Blood Platelets / cytology
  • Case-Control Studies
  • Cell-Derived Microparticles*
  • Complement C3 / analysis
  • Complement C4 / analysis
  • Endothelial Cells / cytology
  • Female
  • Flow Cytometry
  • Humans
  • Leukocytes / cytology
  • Pregnancy


  • Annexins
  • Antibodies, Antiphospholipid
  • Antigens, CD
  • Complement C3
  • Complement C4