The molecular structure of the Corynebacterium glutamicum threonine synthase gene

Mol Microbiol. 1990 Oct;4(10):1693-702. doi: 10.1111/j.1365-2958.1990.tb00546.x.


The minimal region encoding the Corynebacterium glutamicum threonine synthase structural gene and its promoter was mapped by deletion analysis and complementation of the C. glutamicum thrC allele to a 1.6 kb region of the recombinant plasmid pFS80. The nucleotide sequence of this and flanking DNA was determined. The transcription and translation start points were identified by S1 mapping analysis and amino-terminal protein sequencing, respectively. The thrC gene encodes a 54481-Dalton polypeptide product. Translation of the thrC mRNA initiates only six nucleotides downstream from transcription. The length of the mRNA transcript is consistent with a single gene transcription unit. The C. glutamicum thrC gene is expressed independently of the other threonine-specific genes hom and thrB.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Amino Acid Sequence
  • Bacillus subtilis / enzymology
  • Blotting, Northern
  • Carbon-Oxygen Lyases*
  • Corynebacterium / enzymology*
  • Corynebacterium / genetics
  • Escherichia coli / enzymology
  • Genes, Bacterial
  • Lyases / genetics*
  • Molecular Sequence Data
  • Molecular Structure
  • Plasmids
  • Protein Biosynthesis
  • RNA, Messenger / genetics
  • Sequence Homology, Nucleic Acid
  • Transcription, Genetic


  • RNA, Messenger
  • Lyases
  • Carbon-Oxygen Lyases
  • threonine synthase

Associated data

  • GENBANK/X56037