Abstract
MicroRNA profiling of diseased/non-diseased tissue has identified expression signatures associated with a wide range of pathogenic conditions including malignancy. For example, colon cancer is associated with the under expression of miRNA-143 yet the molecular etiology of under expression is unknown. The K-Ras oncogene is a target of miRNA-143. Here, we show that the ecotropic viral integration site 1 oncoprotein (Evi1) is a transcriptional suppressor of the miRNA-143 gene. We find an indirect relationship between miRNA-143 and Evi1 expression. A complex molecular axis linking Evi1, miRNA-143 is operational in human colon cancer.
Copyright © 2011 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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3' Untranslated Regions
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Biomarkers, Tumor / metabolism*
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Cell Line, Tumor
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Cell Migration Assays
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Colonic Neoplasms / metabolism*
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DNA Mutational Analysis
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Gene Expression Regulation, Neoplastic*
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Genes, Reporter
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HEK293 Cells
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Humans
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MDS1 and EVI1 Complex Locus Protein
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MicroRNAs / chemistry
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MicroRNAs / genetics
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MicroRNAs / metabolism*
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Point Mutation
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Proto-Oncogene Proteins p21(ras) / genetics
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Proto-Oncogene Proteins p21(ras) / metabolism*
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Proto-Oncogenes / genetics
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RNA Interference
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RNA, Messenger / metabolism
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Signal Transduction*
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Tissue Banks
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transfection
Substances
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3' Untranslated Regions
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Biomarkers, Tumor
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DNA-Binding Proteins
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MDS1 and EVI1 Complex Locus Protein
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MECOM protein, human
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MIRN143 microRNA, human
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MicroRNAs
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RNA, Messenger
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Transcription Factors
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mirnlet7 microRNA, human
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Proto-Oncogene Proteins p21(ras)