Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion

Nat Med. 2011 Feb;17(2):175-8. doi: 10.1038/nm.2294. Epub 2011 Jan 30.


The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzothiazoles
  • Biomarkers / cerebrospinal fluid
  • Brain / metabolism
  • Creutzfeldt-Jakob Syndrome / cerebrospinal fluid*
  • Creutzfeldt-Jakob Syndrome / diagnosis
  • Fluorescent Dyes
  • Humans
  • Motion
  • PrPSc Proteins / cerebrospinal fluid
  • Prions / cerebrospinal fluid
  • Sensitivity and Specificity
  • Thiazoles


  • Benzothiazoles
  • Biomarkers
  • Fluorescent Dyes
  • PrPSc Proteins
  • Prions
  • Thiazoles
  • thioflavin T