Achromobacter xylosoxidans respiratory tract infection in cystic fibrosis patients

Eur J Clin Microbiol Infect Dis. 2011 Aug;30(8):973-80. doi: 10.1007/s10096-011-1182-5. Epub 2011 Jan 31.


The aims of this study were to evaluate the frequency of Achromobacter xylosoxidans infection in a cohort of cystic fibrosis patients, to investigate antimicrobial sensitivity, to establish possible clonal likeness among strains, and to address the clinical impact of this infection or colonization on the general outcome of these patients. The study was undertaken between January 2004 and December 2008 on 300 patients receiving care at the Regional Cystic Fibrosis Center of the Naples University "Federico II". Sputum samples were checked for bacterial identification. For DNA fingerprinting, pulsed-field gel electrophoresis (PFGE) was carried out. Fifty-three patients (17.6%) had at least one positive culture for A. xylosoxidans; of these, 6/53 (11.3%) patients were defined as chronically infected and all were co-colonized by Pseudomonas aeruginosa. Of the patients, 18.8% persistently carried multidrug-resistant isolates. Macrorestriction analysis showed the presence of seven major clusters. DNA fingerprinting also showed a genetic relationship among strains isolated from the same patients at different times. The results of DNA fingerprinting indicate evidence of bacterial clonal likeness among the enrolled infected patients. We found no significant differences in the forced expiratory volume in 1 s (FEV(1)) and body mass index (BMI) when comparing the case group of A. xylosoxidans chronically infected patients with the control group of P. aeruginosa chronically infected patients.

MeSH terms

  • Achromobacter denitrificans / classification
  • Achromobacter denitrificans / genetics
  • Achromobacter denitrificans / isolation & purification*
  • Adolescent
  • Adult
  • Anti-Bacterial Agents / pharmacology
  • Child
  • Child, Preschool
  • Cluster Analysis
  • Comorbidity
  • Cystic Fibrosis / complications*
  • Electrophoresis, Gel, Pulsed-Field
  • Female
  • Genotype
  • Gram-Negative Bacterial Infections / epidemiology*
  • Gram-Negative Bacterial Infections / microbiology
  • Hospitals
  • Humans
  • Infant
  • Italy / epidemiology
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Molecular Typing
  • Prevalence
  • Pseudomonas Infections / epidemiology
  • Pseudomonas aeruginosa / isolation & purification
  • Respiratory Tract Infections / epidemiology*
  • Respiratory Tract Infections / microbiology
  • Young Adult


  • Anti-Bacterial Agents