Kinetic studies of dextransucrase enzyme reactions on a substrate- or enzyme-immobilized 27 MHz quartz crystal microbalance

Langmuir. 2011 Mar 15;27(6):2107-11. doi: 10.1021/la104550m. Epub 2011 Jan 31.

Abstract

Catalytic elongation by dextransucrase (DSase) was monitored directly on a dextran-acceptor- or DSase-immobilized 27 MHz quartz crystal microbalance (QCM). Kinetic parameters for the binding of the enzyme to the dextran acceptor (k(on), k(off), and K(d)) and enzymatic elongation in the presence of a sucrose monomer (K(m) for sucrose and k(cat)) were determined. The kinetic parameters obtained by both methods were consistent.

MeSH terms

  • Dextrans / chemistry
  • Dextrans / metabolism
  • Enzyme Activation
  • Enzymes, Immobilized / chemistry
  • Enzymes, Immobilized / metabolism*
  • Glucosyltransferases / chemistry
  • Glucosyltransferases / metabolism*
  • Kinetics
  • Quartz Crystal Microbalance Techniques*
  • Substrate Specificity

Substances

  • Dextrans
  • Enzymes, Immobilized
  • Glucosyltransferases
  • dextransucrase