Arachidonic acid stimulates formation of a novel complex containing nucleolin and RhoA

FEBS Lett. 2011 Feb 18;585(4):618-22. doi: 10.1016/j.febslet.2011.01.035. Epub 2011 Feb 1.

Abstract

Arachidonic acid (AA) stimulates cell adhesion through a p38 mitogen activated protein kinase-mediated RhoA signaling pathway. Here we report that a proteomic screen following AA-treatment identified nucleolin, a multifunctional nucleolar protein, in a complex with the GTPase, RhoA, that also included the Rho kinase, ROCK. AA-stimulated cell adhesion was inhibited by expression of nucleolin-targeted shRNA and formation of the multiprotein complex was blocked by expression of dominant-negative RhoA. AA-treatment also induced ROCK-dependent serine phosphorylation of nucleolin and translocation of nucleolin from the nucleus to the cytoplasm, where it appeared to co-localize with RhoA. These data suggest the existence of a new signaling pathway through which the location and post-translational state of nucleolin are modulated.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Amino Acid Substitution
  • Arachidonic Acid / metabolism*
  • Cell Adhesion
  • Cell Line
  • Cell Nucleus / metabolism
  • Cytoplasm / metabolism
  • Gene Silencing
  • Humans
  • Immunoprecipitation
  • Mutant Proteins / genetics
  • Mutant Proteins / metabolism
  • Phosphoproteins / antagonists & inhibitors
  • Phosphoproteins / metabolism*
  • Phosphorylation
  • Protein Kinase Inhibitors
  • Protein Transport
  • Proteomics / methods
  • RNA, Small Interfering / genetics
  • RNA, Small Interfering / metabolism
  • RNA-Binding Proteins / antagonists & inhibitors
  • RNA-Binding Proteins / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Serine / metabolism
  • rho-Associated Kinases / antagonists & inhibitors
  • rho-Associated Kinases / metabolism
  • rhoA GTP-Binding Protein / genetics
  • rhoA GTP-Binding Protein / metabolism*

Substances

  • Mutant Proteins
  • Phosphoproteins
  • Protein Kinase Inhibitors
  • RNA, Small Interfering
  • RNA-Binding Proteins
  • Recombinant Fusion Proteins
  • nucleolin
  • RHOA protein, human
  • Arachidonic Acid
  • Serine
  • rho-Associated Kinases
  • rhoA GTP-Binding Protein