A metabolomics strategy for detecting protein-metabolite interactions to identify natural nuclear receptor ligands

Mol Biosyst. 2011 Apr;7(4):1046-9. doi: 10.1039/c0mb00324g. Epub 2011 Jan 31.


Nuclear receptors are ligand-regulated transcription factors that control a range of (patho)physiological processes. Lipids are the most common type of nuclear receptor ligands. Here, we utilize a metabolomics approach that detects protein-metabolite interactions to identify endogenous peroxisome proliferator-activated receptor (PPAR) ligands from tissue and cell extracts.

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Ligands*
  • Mass Spectrometry
  • Metabolomics*
  • Mice
  • NIH 3T3 Cells
  • Peroxisome Proliferator-Activated Receptors / metabolism
  • Protein Binding
  • Receptors, Cytoplasmic and Nuclear / metabolism*


  • Ligands
  • Peroxisome Proliferator-Activated Receptors
  • Receptors, Cytoplasmic and Nuclear