Positive selection vector using the KillerRed gene

Xuedong Liu; Rui Shi; Dan Zou; Zaiyu Li; Xing Liu; Yanxia Chen; Xiaoguang Yang; Yanna Zhou; Dong Zheng

doi:10.1016/j.ab.2011.01.034

Positive selection vector using the KillerRed gene

Anal Biochem. 2011 May 1;412(1):120-2. doi: 10.1016/j.ab.2011.01.034. Epub 2011 Feb 1.

Authors

Xuedong Liu¹, Rui Shi, Dan Zou, Zaiyu Li, Xing Liu, Yanxia Chen, Xiaoguang Yang, Yanna Zhou, Dong Zheng

Affiliation

¹ Laboratory of Genetics and Molecular Biology, Northeast Forestry University, Harbin 150040, China.

PMID: 21284929
DOI: 10.1016/j.ab.2011.01.034

Abstract

Plasmid pZK18S is a novel positive selection vector containing the genetically encoded photosensitizer KillerRed as the selection marker. When transformed into host cells (lacI(q) or lacI(+)), the common medical surgical light is sufficient to activate phototoxicity of KillerRed. Thus, only the recombinants with disrupted reading frame of KillerRed genes finally allow forming viable colonies. Because lethality of KillerRed relies on light irradiation, no special host and culture medium are required to amplify and prepare pZK18S vector in larger quantities. The pZK18S was reliable and highly efficient for constructing the serial analysis of gene expression (SAGE) library.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Biomarkers / analysis
Biomarkers / chemistry
Cloning, Molecular / methods*
Escherichia coli / metabolism
Genetic Vectors* / chemistry
Genetic Vectors* / isolation & purification
Green Fluorescent Proteins / genetics
Green Fluorescent Proteins / metabolism*
Light
Photosensitizing Agents / chemistry

Substances

Biomarkers
Photosensitizing Agents
killer red protein, Anthomedusae
Green Fluorescent Proteins