The effect of 13C enrichment in the glassing matrix on dynamic nuclear polarization of [1-13C]pyruvate

Phys Med Biol. 2011 Mar 7;56(5):N85-92. doi: 10.1088/0031-9155/56/5/N01. Epub 2011 Feb 1.


Dimethyl sulfoxide (DMSO) can effectively form a glassy matrix necessary for dynamic nuclear polarization (DNP) experiments. We tested the effects of (13)C enrichment in DMSO on DNP of [1-(13)C]pyruvate doped with trityl radical OX063Me. We found that the polarization build-up time τ of pyruvate in (13)C-labeled DMSO glassing solution is twice as fast as the unenriched DMSO while the nuclear magnetic resonance enhancement was unchanged. This indicates that (13)C-(13)C spin diffusion is a limiting factor in the kinetics of DNP in this system, but it has a minimal effect on the absolute value of polarization achievable for the target.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Carbon Isotopes
  • Diffusion
  • Dimethyl Sulfoxide / chemistry
  • Glass / chemistry*
  • Magnetic Resonance Spectroscopy / methods*
  • Pyruvic Acid / chemistry*
  • Water / chemistry


  • Carbon Isotopes
  • Water
  • Pyruvic Acid
  • Dimethyl Sulfoxide