VCre/VloxP and SCre/SloxP: New Site-Specific Recombination Systems for Genome Engineering

Nucleic Acids Res. 2011 Apr;39(8):e49. doi: 10.1093/nar/gkq1280. Epub 2011 Feb 1.


We developed two new site-specific recombination systems named VCre/VloxP and SCre/SloxP for genome engineering. Their recognition sites are different from Cre recognition sites because VCre and SCre recombinases share less protein similarity with Cre, even though the basic 13-8-13 structures of their recognition sites are identical. Mutant VloxP and SloxP, which have the same uses as mutant loxP, were also developed. VCre/VloxP and SCre/SloxP in combination with Cre/loxP and Flp/FRT systems can serve as powerful tools for genome engineering, especially when used to genetically modify both alleles of a single gene in mouse and human cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • DNA / chemistry
  • Embryonic Stem Cells / metabolism
  • Genetic Engineering / methods*
  • Genome
  • HEK293 Cells
  • Humans
  • Mice
  • Mutation
  • Recombinases / metabolism*
  • Recombination, Genetic*


  • Recombinases
  • DNA