A resveratrol and polyphenol preparation suppresses oxidative and inflammatory stress response to a high-fat, high-carbohydrate meal

J Clin Endocrinol Metab. 2011 May;96(5):1409-14. doi: 10.1210/jc.2010-1812. Epub 2011 Feb 2.


Background: High-fat, high-carbohydrate (HFHC) meals are known to induce oxidative and inflammatory stress, an increase in plasma endotoxin concentrations, and an increase in the expression of suppressor of cytokine signaling-3 (SOCS-3).

Hypothesis: The intake of a nutritional supplement containing resveratrol and muscadine grape polyphenols reduces HFHC meal-induced oxidative and inflammatory stress and stimulates the activity of the antioxidant transcription factor, NF-E2-related factor-2 (Nrf-2), and its downstream targets.

Methods: Ten normal, healthy subjects were given a 930-kcal HFHC meal either with placebo or with the supplement. Indices of oxidative stress, inflammation, Nrf-2 binding activity, the concentrations of endotoxin (lipopolysaccharide) and lipoprotein binding protein (LBP), and the expression of toll-like receptor 4 (TLR-4), CD14, IL-1β, TNFα, SOCS-3, Keap-1, NAD(P)H:quinone oxidoreductase-1 (NQO-1), and GST-P1 were measured.

Results: The intake of the supplement suppressed the meal-induced elevations of plasma endotoxin and LBP concentrations, the expression of p47(phox), TLR-4, CD14, SOCS-3, IL-1β, and Keap-1, while enhancing Nrf-2 binding activity and the expression of NQO-1 and GST-P1 genes.

Conclusion: A supplement containing resveratrol and muscadine polyphenols suppresses the increase in oxidative stress, lipopolysaccharide and LBP concentrations, and expression of TLR-4, CD14, IL-1β and SOCS-3 in mononuclear cells after an HFHC meal. It also stimulates specific Nrf-2 activity and induces the expression of the related antioxidant genes, NQO-1 and GST-P1. These results demonstrate the acute antioxidant and antiinflammatory effects of resveratrol and polyphenolic compounds in humans in the postprandial state.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antioxidants / pharmacology*
  • Blotting, Western
  • Cytokines / biosynthesis
  • DNA / biosynthesis
  • DNA / genetics
  • Dietary Carbohydrates / pharmacology*
  • Dietary Fats / adverse effects*
  • Fallopia japonica / chemistry
  • Female
  • Flavonoids / pharmacology*
  • Humans
  • Inflammation / chemically induced
  • Inflammation / metabolism
  • Inflammation / prevention & control*
  • Lipopolysaccharide Receptors / biosynthesis
  • Lipopolysaccharides / pharmacology
  • Male
  • NF-E2-Related Factor 2 / biosynthesis
  • Oxidative Stress / drug effects*
  • Phenols / pharmacology*
  • Plant Extracts / pharmacology
  • Polyphenols
  • Resveratrol
  • Reverse Transcriptase Polymerase Chain Reaction
  • Stilbenes / pharmacology*
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins / biosynthesis
  • Toll-Like Receptor 4 / biosynthesis
  • Vitis / chemistry


  • Antioxidants
  • Cytokines
  • Dietary Carbohydrates
  • Dietary Fats
  • Flavonoids
  • Lipopolysaccharide Receptors
  • Lipopolysaccharides
  • NF-E2-Related Factor 2
  • NFE2L2 protein, human
  • Phenols
  • Plant Extracts
  • Polyphenols
  • SOCS3 protein, human
  • Stilbenes
  • Suppressor of Cytokine Signaling 3 Protein
  • Suppressor of Cytokine Signaling Proteins
  • Toll-Like Receptor 4
  • DNA
  • Resveratrol