Identification of lily pollen 14-3-3 isoforms and their subcellular and time-dependent expression profile

Biol Chem. 2011 Mar;392(3):249-62. doi: 10.1515/BC.2011.026.


14-3-3 proteins are major regulators in plant development and physiology including primary metabolism and signal transduction pathways, typically via a phosphorylation-dependent interaction with a target protein. Four full-length 14-3-3 isoforms were identified in pollen grains of Lilium longiflorum by screening of a cDNA library and RACE (rapid amplification of cDNA ends)-PCR. Mass spectrometry analysis of partially purified 14-3-3s confirmed the presence of the four isoforms but also indicated the presence of additional, less abundant 14-3-3 isoforms in lily pollen. Separation of partially purified 14-3-3 proteins by two-dimensional gel electrophoresis resulted in nine spots that mainly contained the four major 14-3-3 isoforms. In a first step to examine putative physiological roles of specific 14-3-3 isoforms, their subcellular expression profile during pollen germination and tube growth was monitored using a characterized set of antibodies against 14-3-3 proteins with distinct crossreactivity. The abundance profile of 14-3-3 proteins associated with the cytosol, endomembranes (tonoplast, endoplasmic reticulum, Golgi, mitochondria) and plasma membrane showed high spatial-temporal dynamics. This indicates different targets of 14-3-3 proteins at different organelles and time points during pollen germination and growth.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 14-3-3 Proteins / isolation & purification*
  • 14-3-3 Proteins / metabolism*
  • Amino Acid Sequence
  • Gene Library
  • Germination
  • Lilium / growth & development
  • Lilium / metabolism
  • Mass Spectrometry
  • Molecular Sequence Data
  • Organelles
  • Plant Proteins / isolation & purification
  • Plant Proteins / metabolism
  • Pollen / enzymology*
  • Protein Isoforms / analysis
  • Protein Isoforms / isolation & purification
  • Protein Isoforms / metabolism
  • Sequence Alignment / classification


  • 14-3-3 Proteins
  • Plant Proteins
  • Protein Isoforms