The puff-specific Drosophila protein Bj6, encoded by the gene no-on transient A, shows homology to RNA-binding proteins

Chromosoma. 1990 Dec;100(1):37-47. doi: 10.1007/BF00337601.

Abstract

The DNA coding for the puff-specific protein Bj6 has been isolated by expression cloning. The gene is localized in 14C1,2 on the X chromosome and is expressed ubiquitously during embryonic development with prominent expression during the first 12 h of embryogenesis. cDNA and genomic clones have been sequenced and show a single open-reading frame of 2.1 kb length, coding for a Mr = 77,000 basic protein. In the aminoterminal half of the protein we detect stretches of repeated amino acids, centrally a region with homology to RNA-binding proteins containing the RNP 1 and RNP 2 consensus motif of RNA binding proteins, and the carboxyterminal part is rich in charged amino acids. The Bj6 protein is a product of the gene no-on transient A, a gene required for normal vision and courtship behaviour in Drosophila.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / genetics
  • Cloning, Molecular
  • Consensus Sequence
  • Drosophila / embryology
  • Drosophila / genetics*
  • Drosophila Proteins*
  • Molecular Sequence Data
  • Nuclear Proteins / genetics*
  • Nuclear Proteins / metabolism
  • Nucleic Acid Conformation
  • Nucleotide Mapping
  • Open Reading Frames
  • RNA-Binding Proteins
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Sequence Homology, Nucleic Acid
  • X Chromosome*

Substances

  • Carrier Proteins
  • Drosophila Proteins
  • NONA protein, Drosophila
  • Nuclear Proteins
  • RNA-Binding Proteins

Associated data

  • GENBANK/X55902