Carrefour Mme. Gras: a wild-isolated Neurospora crassa strain that suppresses meiotic silencing by unpaired DNA and uncovers a novel ascospore stability defect

Fungal Genet Biol. 2011 Jun;48(6):612-20. doi: 10.1016/j.fgb.2011.01.012. Epub 2011 Feb 2.

Abstract

Ordinarily, RIP-induced erg-3 mutant Neurospora crassa ascospores and their erg(+) siblings do not differ in stability during long-term storage. Consequently, the frequency of RIP-induced erg-3 mutants remains about constant regardless of the time that has elapsed between ascospore harvest and germination. We found, however, that RIP-induced erg-3 mutants were apparently selectively lost with time from among the ascospores stored from a cross with the wild-isolated Carrefour Mme. Gras strain from Haiti. The Haitian strain was also found to exert a dominant suppression of meiotic silencing by unpaired DNA. Similar loss of RIP-induced erg-3 mutant ascospores was seen among the stored ascospores from a subset of crosses heterozygous for the semi-dominant Sad-1 or Sad-2 suppressors of meiotic silencing. Our results suggest that crosses suppressed in meiotic silencing can compromise the stability during storage of ascospores that inherit RIP-induced mutations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Crosses, Genetic
  • DNA, Fungal / genetics*
  • Genes, Mating Type, Fungal
  • Meiosis*
  • Mutation
  • Neurospora crassa / cytology
  • Neurospora crassa / genetics*
  • Neurospora crassa / growth & development
  • Neurospora crassa / isolation & purification
  • Spores, Fungal / cytology*
  • Spores, Fungal / genetics
  • Spores, Fungal / growth & development

Substances

  • DNA, Fungal