Plectin interacts with the rod domain of type III intermediate filament proteins desmin and vimentin

Eur J Cell Biol. 2011 May;90(5):390-400. doi: 10.1016/j.ejcb.2010.11.013. Epub 2011 Feb 5.

Abstract

Plectin is a versatile cytolinker protein critically involved in the organization of the cytoskeletal filamentous system. The muscle-specific intermediate filament (IF) protein desmin, which progressively replaces vimentin during differentiation of myoblasts, is one of the important binding partners of plectin in mature muscle. Defects of either plectin or desmin cause muscular dystrophies. By cell transfection studies, yeast two-hybrid, overlay and pull-down assays for binding analysis, we have characterized the functionally important sequences for the interaction of plectin with desmin and vimentin. The association of plectin with both desmin and vimentin predominantly depended on its fifth plakin repeat domain and downstream linker region. Conversely, the interaction of desmin and vimentin with plectin required sequences contained within the segments 1A-2A of their central coiled-coil rod domain. This study furthers our knowledge of the interaction between plectin and IF proteins important for maintenance of cytoarchitecture in skeletal muscle. Moreover, binding of plectin to the conserved rod domain of IF proteins could well explain its broad interaction with most types of IFs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Desmin / chemistry*
  • Desmin / genetics
  • Desmin / metabolism*
  • Humans
  • Intermediate Filaments / chemistry
  • Mice
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / metabolism
  • Plectin / genetics
  • Plectin / metabolism*
  • Protein Isoforms / chemistry
  • Protein Isoforms / genetics
  • Protein Isoforms / metabolism
  • Protein Structure, Tertiary
  • Rats
  • Recombinant Fusion Proteins / chemistry
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Two-Hybrid System Techniques
  • Vimentin / chemistry*
  • Vimentin / genetics
  • Vimentin / metabolism*

Substances

  • Desmin
  • Plectin
  • Protein Isoforms
  • Recombinant Fusion Proteins
  • Vimentin