Genetic diversity of Imjin virus in the Ussuri white-toothed shrew (Crocidura lasiura) in the Republic of Korea, 2004-2010

Virol J. 2011 Feb 8;8:56. doi: 10.1186/1743-422X-8-56.

Abstract

Recently, Imjin virus (MJNV), a genetically distinct hantavirus, was isolated from lung tissues of the Ussuri white-toothed shrew (Crocidura lasiura) captured near the demilitarized zone in the Republic of Korea. To clarify the genetic diversity of MJNV, partial M- and L-segment sequences were amplified from lung tissues of 12 of 37 (32.4%) anti-MJNV IgG antibody-positive Ussuri white-toothed shrews captured between 2004 and 2010. A 531-nucleotide region of the M segment (coordinates 2,255 to 2,785) revealed that the 12 MJNV strains differed by 0-12.2% and 0-2.3% at the nucleotide and amino acid levels, respectively. A similar degree of nucleotide (0.2-11.9%) and amino acid (0-3.8%) difference was found in a 632-nucleotide length of the L segment (coordinates 962 to 1,593) of nine MJNV strains. Phylogenetic analyses, based on the partial M and L segments of MJNV strains generated by the neighbor-joining and maximum likelihood methods, showed geographic-specific clustering, akin to the phylogeography of rodent-borne hantaviruses.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Genetic Variation*
  • Hantavirus / classification*
  • Hantavirus / genetics*
  • Hantavirus / isolation & purification
  • Insectivora / virology*
  • Lung / virology
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Viral / genetics
  • Republic of Korea
  • Sequence Analysis, DNA
  • Sequence Homology
  • Viral Proteins / genetics

Substances

  • RNA, Viral
  • Viral Proteins

Associated data

  • GENBANK/HQ201404
  • GENBANK/HQ201405
  • GENBANK/HQ201406
  • GENBANK/HQ201407
  • GENBANK/HQ201408
  • GENBANK/HQ201409
  • GENBANK/HQ201410
  • GENBANK/HQ201411
  • GENBANK/HQ201412
  • GENBANK/HQ201413
  • GENBANK/HQ201414
  • GENBANK/HQ201415