Synthesis of novel tricalcium phosphate-bioactive glass composite and functionalization with rhBMP-2

J Mater Sci Mater Med. 2011 Apr;22(4):763-71. doi: 10.1007/s10856-011-4252-4. Epub 2011 Feb 10.


A functionalization is required for calcium phosphate-based bone substitute materials to achieve an entire bone remodeling. In this study it was hypothesized that a tailored composite of tricalcium phosphate and a bioactive glass can be loaded sufficiently with rhBMP-2 for functionalization. A composite of 40 wt% tricalcium phosphate and 60 wt% bioactive glass resulted in two crystalline phases, wollastonite and rhenanite after sintering. SEM analysis of the composite's surface revealed a spongious bone-like morphology after treatment with different acids. RhBMP-2 was immobilized non-covalently by treating with chrome sulfuric acid (CSA) and 3-aminopropyltriethoxysilane (APS) and covalently by treating with CSA/APS, and additionally with 1,1'-carbonyldiimidazole. It was proved that samples containing non-covalently immobilized rhBMP-2 on the surface exhibit significant biological activity in contrast to the samples with covalently bound protein on the surface. We conclude that a tailored composite of tricalcium phosphate and bioactive glass can be loaded sufficiently with BMP-2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Bone Morphogenetic Protein 2 / chemistry
  • Bone Remodeling
  • Bone Substitutes / chemistry*
  • Bone and Bones / metabolism
  • Calcium Phosphates / chemistry*
  • Glass / chemistry*
  • Humans
  • Imidazoles / chemistry
  • Materials Testing
  • Mice
  • Microscopy, Electron, Scanning / methods
  • Propylamines
  • Recombinant Proteins / chemistry
  • Silanes / chemistry
  • Sulfuric Acids / chemistry
  • Surface Properties
  • X-Ray Diffraction


  • Bone Morphogenetic Protein 2
  • Bone Substitutes
  • Calcium Phosphates
  • Imidazoles
  • Propylamines
  • Recombinant Proteins
  • Silanes
  • Sulfuric Acids
  • N,N-carbonyldiimidazole
  • tricalcium phosphate
  • amino-propyl-triethoxysilane
  • sulfuric acid