Ly6C supports preferential homing of central memory CD8+ T cells into lymph nodes

Eur J Immunol. 2011 Mar;41(3):634-44. doi: 10.1002/eji.201040760. Epub 2011 Feb 10.


Ly6C is a murine cell-surface antigen expressed by plasma cells, subsets of myeloid cells and many T cells, including memory T cells. We previously documented that Ly6C crosslinking induces LFA-1 clustering on naïve CD8(+) T cells. Here, we show that in vitro and in vivo differentiation of naïve CD8(+) T cells into central (Tcm) but not effector (Tem) memory T cells enhances Ly6C expression, and its crosslinking induces strong LFA-1 clustering on Tcm. Blocking Ly6C function inhibits in vivo Tcm homing to LNs as efficiently as blocking L-selectin but it does not potentiate the inhibition provided by blocking either L-selectin or LFA-1 function. Thus, Ly6C, L-selectin and LFA-1 all appear to be part of a common homing pathway. In vitro, Ly6C crosslinking enhances Tcm adherence to ICAM-1 in the presence of CCL21. In summary, Tcm homing involves Ly6C, in addition to L-selectin and LFA-1, and appears to potentiate firm adhesion of Tcm to ICAM-1 in synergy with a chemokine. We propose that Ly6C augments Tcm compartmentalization into LNs during their homing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antigens, Ly / chemistry
  • Antigens, Ly / metabolism*
  • CD8-Positive T-Lymphocytes / cytology
  • CD8-Positive T-Lymphocytes / immunology*
  • Cell Differentiation / immunology
  • Cell Line
  • Cell Movement / immunology
  • Cross-Linking Reagents
  • Immunologic Memory
  • In Vitro Techniques
  • L-Selectin / metabolism
  • Liver / cytology
  • Liver / immunology
  • Lymphocyte Function-Associated Antigen-1 / metabolism
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Receptors, Lymphocyte Homing / metabolism
  • Signal Transduction / immunology
  • Up-Regulation


  • Antigens, Ly
  • Cross-Linking Reagents
  • Ly-6C antigen, mouse
  • Lymphocyte Function-Associated Antigen-1
  • Receptors, Lymphocyte Homing
  • L-Selectin