Non-invasive screening of HLA-DPA1 and HLA-DPB1 alleles for persistent hepatitis B virus infection: susceptibility for vertical transmission and toward a personalized approach for vaccination and treatment

Clin Chim Acta. 2011 May 12;412(11-12):952-7. doi: 10.1016/j.cca.2011.01.030. Epub 2011 Feb 16.


Background: Polymorphisms in the major histocompatibility complex (MHC) and non-MHC genes were recently reported to be associated with persistent hepatitis B virus (HBV) infection and host response to hepatitis B vaccine in Asian populations. We aimed to confirm the associations in Chinese population and develop a non-invasive screening method for the risk loci.

Methods: We genotyped 2 risk alleles on the MHC loci, HLA-DPA1 (rs3077) and HLA-DPB1 (rs9277535), and 1 risk allele near a non-MHC gene, FOXP1 (rs6789153) using high-resolution melting curve analysis. With minimal processing steps and time, salivary DNA was extracted with a modified protocol of a blood kit. We compared the genotyping fidelity between peripheral blood DNA and salivary DNA.

Results: Both rs3077 and rs9277535, but not rs6789153, are significantly associated with CHB in Chinese population (p-value<0.001). High genotype concordance between different sources of genomic DNA was obtained.

Conclusions: Genotyping salivary DNA using our modified methods provides a non-invasive fast screening for host susceptibility loci. The transmission mechanism of hepatitis B can now be modified by adding genetic susceptibility to the traditional vertical transmission model of hepatitis B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Alleles*
  • Female
  • Genetic Loci / genetics
  • Genetic Testing / methods*
  • HLA-DP alpha-Chains / genetics
  • HLA-DP beta-Chains / genetics
  • Hepatitis B virus / pathogenicity
  • Hepatitis B, Chronic / genetics*
  • Hepatitis B, Chronic / prevention & control
  • Hepatitis B, Chronic / therapy
  • Hepatitis B, Chronic / transmission*
  • Humans
  • Infectious Disease Transmission, Vertical*
  • Male
  • Middle Aged
  • Precision Medicine / methods*
  • Vaccination / methods*
  • Young Adult


  • HLA-DP alpha-Chains
  • HLA-DP beta-Chains
  • HLA-DPA1 antigen
  • HLA-DPB1 antigen