Ani s 10, a new Anisakis simplex allergen: cloning and heterologous expression

Parasitol Int. 2011 Jun;60(2):209-12. doi: 10.1016/j.parint.2011.01.003. Epub 2011 Feb 16.

Abstract

Anisakiasis is a human disease caused by accidental ingestion of larval nematodes belonging to the Anisakidae family. Anisakiasis is often associated with a strong allergic response. Diagnosis of A. simplex allergy is currently carried out by test based on the IgE reactivity to a complete extract of L3 Anisakis larvae although the specificity of these diagnostic tests is poor. Improving the specificity of the diagnostic test is possible using purified recombinant allergens. A new Anisakis allergen, named Ani s 10, was detected by immunoscreening an expression cDNA library constructed from L3 Anisakis simplex larvae. The new allergen was overproduced in Escherichia coli; it is a protein of 212 amino acids and it was localized as a 22 kDa protein band in an ethanol fractionated extract from the parasite. Ani s 10 has no homology with any other described protein, and its sequence is composed by seven almost identical repetitions of 29 amino acids each. A total of 30 of 77 Anisakis allergic patients (39%) were positive both to rAni s 10 and natural Ani s 10 by immunoblotting. The new allergen could be useful in a component-resolved diagnosis system for Anisakis allergy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Allergens / chemistry*
  • Allergens / immunology
  • Amino Acid Sequence
  • Animals
  • Anisakiasis / immunology
  • Anisakis / genetics*
  • Anisakis / immunology
  • Antibodies, Helminth / blood
  • Antigens, Helminth / genetics*
  • Antigens, Helminth / immunology
  • Base Sequence
  • Cloning, Molecular
  • DNA, Complementary / metabolism
  • DNA, Helminth / genetics
  • Escherichia coli / metabolism
  • Female
  • Gene Library
  • Helminth Proteins / genetics
  • Helminth Proteins / immunology
  • Humans
  • Hypersensitivity / immunology
  • Hypersensitivity / parasitology
  • Immunoblotting
  • Immunoglobulin E / blood
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Plasmids
  • Sequence Analysis, DNA
  • Young Adult

Substances

  • Allergens
  • Antibodies, Helminth
  • Antigens, Helminth
  • DNA, Complementary
  • DNA, Helminth
  • Helminth Proteins
  • Immunoglobulin E

Associated data

  • GENBANK/GU187358