Environmental Burkholderia cepacia strain Cs5 acting by two analogous alkyl-quinolones and a didecyl-phthalate against a broad spectrum of phytopathogens fungi

Curr Microbiol. 2011 May;62(5):1490-5. doi: 10.1007/s00284-011-9892-6. Epub 2011 Feb 11.


An environmental Burkholderia cepacia strain named Cs5 was isolated and identified first using API biochemical identification system and then with 16S rDNA and recA sequence homology search. This bacterium exhibited a broad spectrum of fungicidal activities against Alternaria alternata, Aspergillus niger, Fusarium culmorum, F. graminearum, F. oxysporum and Rhizoctonia solani. In the liquid conditions, the MIC of A. niger and R. solani were reached with, respectively, 1.25-2% of the Cs5 liquid culture supernatant. However, in the solid conditions, the same inhibition was caused in the presence of 3% of the Cs5 supernatant. The exhibition of these two fungi at low concentrations of supernatant Cs5 caused various morphological changes of their mycelia which were observed by confocal microscopy. Three antifungal compounds, named Cs5-255, Cs5-257 and Cs5-446, were purified from the Cs5 culture. The structural analysis of these molecules showed that Cs5-255 and Cs5-257 are analogous and belonged to the alkyl-quinolone family, while Cs5-446 was a didecyl-phthalate, isolated for the first time from a bacterium.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antifungal Agents / chemistry
  • Antifungal Agents / isolation & purification
  • Antifungal Agents / metabolism*
  • Antifungal Agents / pharmacology
  • Burkholderia cepacia / chemistry
  • Burkholderia cepacia / genetics
  • Burkholderia cepacia / isolation & purification
  • Burkholderia cepacia / metabolism*
  • Fungi / drug effects*
  • Fungi / growth & development
  • Fungi / physiology
  • Molecular Structure
  • Phthalic Acids / chemistry
  • Phthalic Acids / isolation & purification
  • Phthalic Acids / metabolism*
  • Phthalic Acids / pharmacology
  • Plant Diseases / microbiology*
  • Prunus / microbiology
  • Quinolones / chemistry
  • Quinolones / isolation & purification
  • Quinolones / metabolism*
  • Quinolones / pharmacology


  • Antifungal Agents
  • Phthalic Acids
  • Quinolones
  • didecyl phthalate