Abstract
Injection of endothelial progenitor cells (EPCs) into arteries for cell therapy is a promising field in regenerative medicine. However, adhesion of EPCs during capillary passage is restricted, and non-adhering cells are lost into circulation. Here we demonstrate that it is possible to achieve a three- to sevenfold higher rate of EPC adhesion to endothelium and extracellular matrix molecules after short-term activation with phorbol myristate acetate (PMA). In addition, differentiation and toxicity analyses of PMA activated EPCs showed no impact on cell differentiation and negligible impact on cell survival.
MeSH terms
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Apoptosis / drug effects
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Biomarkers / metabolism
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Cell Adhesion / drug effects
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Cell Differentiation / drug effects
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Collagen / metabolism
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Dose-Response Relationship, Drug
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Endothelium, Vascular / cytology*
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Extracellular Matrix / drug effects*
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Extracellular Matrix / metabolism*
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Fibronectins / metabolism
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Gene Expression Regulation / drug effects
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Humans
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Laminin / metabolism
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Necrosis / chemically induced
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Stem Cells / cytology*
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Stem Cells / drug effects*
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Stem Cells / metabolism
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Tetradecanoylphorbol Acetate / pharmacology*
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Time Factors
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Umbilical Veins / cytology
Substances
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Biomarkers
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Fibronectins
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Laminin
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Collagen
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Tetradecanoylphorbol Acetate