Peroxiredoxin Stabilization of DE-cadherin Promotes Primordial Germ Cell Adhesion

Dev Cell. 2011 Feb 15;20(2):233-43. doi: 10.1016/j.devcel.2010.12.007.

Abstract

Regulated adhesion between cells and their environment is critical for normal cell migration. We have identified mutations in a gene encoding the Drosophila hydrogen peroxide (H₂O₂)-degrading enzyme Jafrac1, which lead to germ cell adhesion defects. During gastrulation, primordial germ cells (PGCs) associate tightly with the invaginating midgut primordium as it enters the embryo; however, in embryos from jafrac1 mutant mothers this association is disrupted, leaving some PGCs trailing on the outside of the embryo. We observed similar phenotypes in embryos from DE-cadherin/shotgun (shg) mutant mothers and were able to rescue the jafrac1 phenotype by increasing DE-cadherin levels. This and our biochemical evidence strongly suggest that Jafrac1-mediated reduction of H₂O₂ is required to maintain DE-cadherin protein levels in the early embryo. Our results present in vivo evidence of a peroxiredoxin regulating DE-cadherin-mediated adhesion.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adherens Junctions / drug effects
  • Adherens Junctions / metabolism
  • Animals
  • Cadherins / genetics
  • Cadherins / metabolism*
  • Cell Adhesion / drug effects
  • Cell Aggregation / drug effects
  • Cell Survival / drug effects
  • Drosophila Proteins / genetics
  • Drosophila Proteins / metabolism*
  • Drosophila melanogaster / cytology*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / enzymology*
  • Drosophila melanogaster / metabolism
  • Embryo, Nonmammalian / cytology
  • Embryo, Nonmammalian / drug effects
  • Embryo, Nonmammalian / metabolism
  • Endocytosis / drug effects
  • Gastrulation / drug effects
  • Gene Expression Regulation / drug effects
  • Germ Cells / cytology*
  • Germ Cells / drug effects
  • Germ Cells / enzymology*
  • Hydrogen Peroxide / pharmacology
  • Oxidative Stress / drug effects
  • Peroxidases / metabolism*
  • Protein Stability / drug effects
  • Transcription, Genetic / drug effects

Substances

  • Cadherins
  • Drosophila Proteins
  • shg protein, Drosophila
  • Hydrogen Peroxide
  • Jafrac1 protein, Drosophila
  • Peroxidases