Characterization of a novel long-chain n-alkane-degrading strain, Dietzia sp. E1

Z Naturforsch C J Biosci. 2010 Nov-Dec;65(11-12):693-700. doi: 10.1515/znc-2010-11-1210.

Abstract

The newly isolated strain E1, identified as a Dietzia sp., proved to have an excellent ability to degrade n-C12 to n-C38 alkane components of crude oil. The preferred substrate was the very long-chain alkane n-eicosane at an optimal temperature of 37 degrees C and an optimal pH of 8 under aerobic conditions. The growth and substrate uptake kinetics were monitored during the n-alkane fermentation process, and Dietzia sp. E1 cells were found to possess three distinct levels of cell-surface hydrophobicity. Gas chromatographic/mass spectrometric analysis revealed that intracellular substrate mineralization occurred through the conversion of n-alkane to the corresponding n-alkanal. The monoterminal oxidation pathway was presumably initiated by AlkB and CYP153 terminal alkane hydroxylases, both of their partial coding sequences were successfully detected in the genome of strain E1, a novel member of the Dietzia genus.

MeSH terms

  • Actinobacteria / genetics
  • Actinobacteria / growth & development
  • Actinobacteria / metabolism*
  • Alkanes / metabolism*
  • Biodegradation, Environmental
  • Cytochrome P-450 CYP4A / genetics
  • Gas Chromatography-Mass Spectrometry

Substances

  • Alkanes
  • Cytochrome P-450 CYP4A