Structural analysis and activation by fungal infection of a gene encoding a pathogenesis-related protein in potato

Mol Plant Microbe Interact. Mar-Apr 1990;3(2):72-7.

Abstract

The structure, genomic organization, and temporal pattern of activation of a gene encoding a pathogenesis-related protein (PR1) in potato (Solanum tuberosum) have been analyzed. The gene is rapidly activated in leaves from the potato cultivar Datura, containing the resistance gene R1, in both compatible and incompatible interactions with appropriate races of the late-blight fungus Phytophthora infestans. Activation is also observed in leaves treated with fungal elicitor. The gene occurs in multiple, very similar copies and encodes a polypeptide (Mr = 25,054; pI = 5.5) that is classified as a PR protein by several criteria. Small fragments with great sequence similarity to portions of the two exons were found closely linked to the expressed gene, which altogether represents a simple case of genome organization in potato. The coding sequence of the prp1 gene and the deduced amino acid sequence are strikingly similar to the corresponding sequences of a 26-kDa heat shock protein from soybean.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA
  • Gene Expression Regulation*
  • Heat-Shock Proteins / genetics
  • Kinetics
  • Molecular Sequence Data
  • Phytophthora / physiology*
  • Plant Diseases
  • Plant Proteins / genetics*
  • Protein Biosynthesis
  • RNA, Messenger / metabolism
  • Restriction Mapping
  • Sequence Alignment
  • Solanum tuberosum / genetics*
  • Solanum tuberosum / microbiology

Substances

  • Heat-Shock Proteins
  • Plant Proteins
  • RNA, Messenger
  • pathogenesis-related proteins, plant
  • DNA

Associated data

  • GENBANK/J03679