The temporal relationship between non-respiratory burst activity of expiratory laryngeal motoneurons and phrenic apnoea during stimulation of the superior laryngeal nerve in rat

J Physiol. 2011 Apr 1;589(Pt 7):1819-30. doi: 10.1113/jphysiol.2010.203794. Epub 2011 Feb 14.


A striking effect of stimulating the superior laryngeal nerve (SLN) is its ability to inhibit central inspiratory activity (cause ‘phrenic apnoea'), but the mechanism underlying this inhibition remains unclear. Here we demonstrate, by stimulating the SLN at varying frequencies, that the evoked non-respiratory burst activity recorded from expiratory laryngeal motoneurons (ELMs) has an intimate temporal relationship with phrenic apnoea. During 1–5 Hz SLN stimulation, occasional absences of phrenic nerve discharge (PND) occurred such that every absent PND was preceded by an ELM burst activity. During 10–20 Hz SLN stimulation, more bursts were evoked together with more absent PNDs, leading eventually to phrenic apnoea. Interestingly, subsequent microinjections of isoguvacine (10 mm, 20–40 nl) into ipsilateral Bötzinger complex (BötC) and contralateral nucleus tractus solitarii (NTS) significantly attenuated the apnoeic response but not the ELM burst activity. Our results suggest a bifurcating projection from NTS to both the caudal nucleus ambiguus and BötC, which mediates the closely related ELM burst and apnoeic response, respectively. We believe that such an intimate timing between laryngeal behaviour and breathing is crucial for the effective elaboration of the different airway protective behaviours elicited following SLN stimulation, including the laryngeal adductor reflex, swallowing and cough.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apnea / physiopathology
  • Cough / physiopathology
  • Deglutition / physiology
  • Electric Stimulation
  • Electrophysiological Phenomena
  • Evoked Potentials
  • Exhalation / physiology*
  • Humans
  • Isonicotinic Acids / administration & dosage
  • Laryngeal Nerves / physiology*
  • Larynx / physiology*
  • Male
  • Microinjections
  • Models, Animal
  • Models, Neurological
  • Motor Neurons / physiology*
  • Phrenic Nerve / physiology
  • Rats
  • Rats, Sprague-Dawley
  • Reflex / physiology
  • Respiratory Aspiration / physiopathology
  • Respiratory Mechanics / physiology


  • Isonicotinic Acids
  • isoguvacine