Genomic analysis of a pneumovirus isolated from dogs with acute respiratory disease

Vet Microbiol. 2011 May 12;150(1-2):88-95. doi: 10.1016/j.vetmic.2011.01.013. Epub 2011 Jan 15.


A previously unrecognized virus belonging to the subfamily Pneumovirinae and most closely related to murine pneumovirus (MPV) was identified in domestic dogs in 2 related animal shelters. Additional diagnostic testing yielded 3 new viral isolates and identified 6 additional PCR positive dogs from other USA locations indicating that its distribution is not geographically limited. Nucleotide sequences encompassing 9 of the 10 genes were compared to the only 2 available MPV strains, 15 and J3666. Several features distinguished the canine pneumovirus (CnPnV) from the murine strains. Two regions of diversity were identified in the amino-proximal region of P and the overlapping P2 ORF was only 54 amino acids (aa) compared to 137aa in MPV. The G protein had an amino-terminal cytoplasmic tail 18aa longer than in the MPV strains. The CnPnV SH protein showed the highest divergence with only 90.2% aa identity when compared to MPV strain 15. Like strain 15, the CnPnV SH ORF coded for a protein of 92aa while J3666 has a 114aa variant. Comparison of CnPnV isolates at culture passages 4 and 17 revealed 7nt differences within the 8598nt sequenced. Of note was a substitution at nt 364 in G resulting in a termination codon that would produce a truncated G protein of 122aa. Analysis of early passage and ex vivo samples showed the termination codon in G to be predominant after 6 days in culture indicating rapid selection of the mutation in A72 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Dog Diseases / virology*
  • Dogs / virology*
  • Genome, Viral*
  • Genomics
  • Open Reading Frames
  • Phylogeny
  • Pneumovirus / classification
  • Pneumovirus / genetics*
  • Pneumovirus / isolation & purification
  • Pneumovirus Infections / veterinary*
  • Pneumovirus Infections / virology
  • RNA, Viral / genetics
  • Sequence Analysis, RNA
  • Sequence Homology, Amino Acid
  • United States
  • Viral Proteins / genetics


  • RNA, Viral
  • Viral Proteins