Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers

Glycobiology. 2011 Jul;21(7):895-902. doi: 10.1093/glycob/cwr021. Epub 2011 Feb 16.


Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrylic Resins / metabolism
  • Antibodies / chemistry
  • Antibodies / metabolism*
  • Humans
  • Lectins / chemistry
  • Lectins / metabolism*
  • Microarray Analysis
  • Photosensitizing Agents*
  • Polysaccharides / chemistry
  • Polysaccharides / metabolism*
  • Protein Binding
  • Proteins / chemistry
  • Proteins / metabolism*


  • Acrylic Resins
  • Antibodies
  • Lectins
  • Photosensitizing Agents
  • Polysaccharides
  • Proteins
  • polyacrylamide