I(h)-mediated depolarization enhances the temporal precision of neuronal integration

Nat Commun. 2011 Feb 15:2:199. doi: 10.1038/ncomms1202.

Abstract

Feed-forward inhibition mediated by ionotropic GABA(A) receptors contributes to the temporal precision of neuronal signal integration. These receptors exert their inhibitory effect by shunting excitatory currents and by hyperpolarizing neurons. The relative roles of these mechanisms in neuronal computations are, however, incompletely understood. In this study, we show that by depolarizing the resting membrane potential relative to the reversal potential for GABA(A) receptors, the hyperpolarization-activated mixed cation current (I(h)) maintains a voltage gradient for fast synaptic inhibition in hippocampal pyramidal cells. Pharmacological or genetic ablation of I(h) broadens the depolarizing phase of afferent synaptic waveforms by hyperpolarizing the resting membrane potential. This increases the integration time window for action potential generation. These results indicate that the hyperpolarizing component of GABA(A) receptor-mediated inhibition has an important role in maintaining the temporal fidelity of coincidence detection and suggest a previously unrecognized mechanism by which I(h) modulates information processing in the hippocampus.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cations / metabolism*
  • Computer Simulation
  • Cyclic Nucleotide-Gated Cation Channels / metabolism*
  • Electrophysiology
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Male
  • Membrane Potentials / physiology*
  • Models, Biological
  • Potassium Channels / metabolism*
  • Pyramidal Cells / physiology*
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, GABA-A / metabolism*
  • Synaptic Transmission / physiology*

Substances

  • Cations
  • Cyclic Nucleotide-Gated Cation Channels
  • Hyperpolarization-Activated Cyclic Nucleotide-Gated Channels
  • Potassium Channels
  • Receptors, GABA-A