Stiffness of photocrosslinked RGD-alginate gels regulates adipose progenitor cell behavior

Biotechnol Bioeng. 2011 Jul;108(7):1683-92. doi: 10.1002/bit.23079. Epub 2011 Feb 24.


Adipose progenitor cells (APCs) are widely investigated for soft tissue reconstruction following tumor resection; however, the long-term success of current approaches is still limited. In order to develop clinically relevant therapies, a better understanding of the role of cell-microenvironment interactions in adipose tissue regeneration is essential. In particular, the effect of extracellular matrix (ECM) mechanics on the regenerative capability of APCs remains to be clarified. We have used artificial ECMs based on photocrosslinkable RGD-alginate to investigate the adipogenic and pro-angiogenic potential of 3T3-L1 preadipocytes as a function of matrix stiffness. These hydrogels allowed us to decouple matrix stiffness from changes in adhesion peptide density or extracellular Ca(2+) concentration and provided a physiologically relevant 3D culture context. Our findings suggest that increased matrix rigidity promotes APC self-renewal and angiogenic capacity, whereas, it inhibits adipose differentiation. Collectively, this study advances our understanding of the role of ECM mechanics in adipose tissue formation and vascularization and will aid in the design of efficacious biomaterial scaffolds for adipose tissue engineering applications.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adipose Tissue*
  • Alginates / chemistry*
  • Alginates / radiation effects*
  • Glucuronic Acid / chemistry
  • Glucuronic Acid / radiation effects
  • Hexuronic Acids / chemistry
  • Hexuronic Acids / radiation effects
  • Hydrogel, Polyethylene Glycol Dimethacrylate / chemistry
  • Hydrogel, Polyethylene Glycol Dimethacrylate / radiation effects
  • Light
  • Stem Cells / physiology*
  • Tissue Engineering / methods*


  • Alginates
  • Hexuronic Acids
  • Hydrogel, Polyethylene Glycol Dimethacrylate
  • Glucuronic Acid