IgE and IgG(4) autoantibodies against DFS70/LEDGF in atopic dermatitis

Autoimmunity. 2011 Sep;44(6):511-9. doi: 10.3109/08916934.2010.549157. Epub 2011 Feb 18.

Abstract

Background: DFS70/LEDGF is a known survival factor, growth factor, and HIV-1 transporter. It is also an autoantigen recognized by IgG autoantibodies (aAbs) in patients with atopic dermatitis (AD). In the past few decades, various IgE aAbs against self-proteins have been identified in AD. Although DFS70 is considered to be one of these proteins, to date, there has not been a detailed analysis of the significance of this putative autoantigen.

Objective and methods: Using recombinant protein that was generated using a baculovirus and insect cell system, we established an ELISA that can measure IgE-anti-DFS70 aAbs and IgG(4)-anti-DFS70 aAbs. Sera obtained from 61 AD patients and 20 healthy donors were analyzed using the ELISA.

Results: Nine of 61 AD patients were determined to be IgE-anti-DFS70 aAb-positive. IgG(4)-anti-DFS70 aAbs were detected in nine of 61 patients. The serum thymus and activation-regulated chemokine concentrations were significantly higher in the group that was positive for both aAbs compared to the aAb-negative group, although the total serum IgE levels were not significantly different between these groups.

Conclusion: We conclude that serum IgE-anti-DFS70 aAbs and IgG(4)-anti-DFS70 aAbs may be related to the severity of AD.

MeSH terms

  • Adaptor Proteins, Signal Transducing / immunology*
  • Adolescent
  • Adult
  • Autoantibodies / blood*
  • Autoantibodies / immunology
  • Chemokines / blood
  • Dermatitis, Atopic / immunology*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Humans
  • Immunoglobulin E / immunology*
  • Immunoglobulin G / immunology*
  • Intercellular Signaling Peptides and Proteins / immunology
  • Male
  • Middle Aged
  • Transcription Factors / immunology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Autoantibodies
  • Chemokines
  • Immunoglobulin G
  • Intercellular Signaling Peptides and Proteins
  • PSIP1 protein, human
  • Transcription Factors
  • Immunoglobulin E