[Cisplatin-induced Apoptosis in Human Lung Adenocarcinoma Cell Line A549]

Zhongguo Fei Ai Za Zhi. 2002 Aug 20;5(4):254-6. doi: 10.3779/j.issn.1009-3419.2002.04.04.
[Article in Chinese]


Background: To elucidate the pattern and mechanism of cisplatin-induced apoptosis and its role in tumor chemotherapy.

Methods: Apoptosis induced by cisplatin in human lung adenocarcinoma cell line A549 was detected by cell morphology, agarose gel electrophoresis, DNA-end-labeling and flow cytometry analysis techniques.

Results: Cisplatin-induced apoptosis of A549 cells persisted and augmented gradually from 12 to 72 hours after treated with 3 mg/L cisplatin. All of A549 cells treated respectively with 1, 3, 5 and 7 mg/L cisplatin showed apoptosis. Apoptotic effects increased in a time-dependent pattern and a concentration-dependent pattern. A549 cells were blocked in G1 phase after treated with cisplatin.

Conclusions: Induction of cell apoptosis may be an important mechanism of anti-tumor efficacy of cisplatin.

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  • English Abstract