Male Wistar rats were subjected to daily sessions of electrical amygdala kindling until a generalized seizure was observed on five consecutive days. Bilateral microinjections (0.5 microliter) of ethosuximide (ETX) (10 pg/microliters) or saline were then administered through guide cannulas into the substantia nigra (pars reticulata). No significant difference was observed between the ETX (N = 8) and saline (N = 8) groups in duration of afterdischarge or in the latency for stage 5 generalized seizures. Our results indicate that ETX applied to the substantia nigra is not effective in suppressing amygdala-kindled seizures.