15-hydroxyeicosatetraenoic acid synthesis from exogenous arachidonate by bronchial mucosa

J Lipid Mediat. Sep-Oct 1990;2(5):263-9.

Abstract

Arachidonate metabolism in bronchial mucosa was investigated. Bronchial biopsies from four asthmatics and three non-asthmatic controls were challenged with 300, 465, 650 and 960 mOsm/kg saline in the presence of [3H]arachidonate. Supernatants were analysed by reverse-phase high-performance liquid chromatography. The major arachidonate metabolite was identified as 15-monohydroxyeicosatetraenoic acid (15-HETE), with significantly higher production in biopsies from asthmatics than controls. Statistical analysis of the full model explained 81% of the variation (F = 14.8; df = 6, 21; P less than 0.001). Hyperosmolarity and the presence of sarcoid had no significant effect on 15-HETE synthesis, reducing R2 by only 6%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Arachidonic Acid / metabolism*
  • Asthma / metabolism
  • Bronchi / metabolism*
  • Humans
  • Hydroxyeicosatetraenoic Acids / biosynthesis*
  • In Vitro Techniques
  • Middle Aged
  • Mucous Membrane / metabolism

Substances

  • Hydroxyeicosatetraenoic Acids
  • Arachidonic Acid
  • 15-hydroxy-5,8,11,13-eicosatetraenoic acid