Cytokine profiles in aqueous humor of patients with different clinical entities of endogenous uveitis

Clin Immunol. 2011 May;139(2):177-84. doi: 10.1016/j.clim.2011.01.014. Epub 2011 Jan 31.

Abstract

We assayed aqueous humor (AH) samples from patients with Behçet's disease (BD), Vogt-Koyanagi-Harada (VKH) disease, and HLA-B27-associated uveitis and control patients for the proinflammatory cytokines IL-15, IL-17, interferon-γ and tumor necrosis factor-α and the immunosuppressive cytokine IL-10. Cytokine levels were significantly higher in the three disease groups than in controls. In patients with similar disease activity, levels of IL-15 and IFN-γ were significantly higher in BD patients than in VKH and HLA-B27-associated uveitis groups. Logistic regression identified a significant negative correlation between BD and high levels of IL-10 and a significant positive correlation between VKH disease and high levels of IL-10. The proinflammatory cytokines versus IL-10 ratios were significantly higher in BD compared with other groups. These data suggest that both T helper (Th) 1 and Th17 cells are involved in endogenous uveitis immunopathogenesis. BD is characterized by extensive Th1 polarization, severe proinflammatory conditions and a low immunosuppressive status.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adrenal Cortex Hormones / administration & dosage
  • Adrenal Cortex Hormones / pharmacology
  • Adrenal Cortex Hormones / therapeutic use
  • Adult
  • Aqueous Humor / drug effects
  • Aqueous Humor / metabolism*
  • Behcet Syndrome / diagnosis
  • Behcet Syndrome / drug therapy
  • Behcet Syndrome / immunology
  • Behcet Syndrome / metabolism
  • Cyclosporine / administration & dosage
  • Cyclosporine / pharmacology
  • Cyclosporine / therapeutic use
  • Cytokines / metabolism*
  • Female
  • HLA-B27 Antigen / genetics
  • Humans
  • Interferon-gamma / metabolism
  • Interleukin-10 / metabolism
  • Interleukin-15 / metabolism
  • Interleukin-17 / metabolism
  • Logistic Models
  • Male
  • Middle Aged
  • Th1 Cells / immunology
  • Th1 Cells / metabolism
  • Th17 Cells / immunology
  • Th17 Cells / metabolism
  • Treatment Outcome
  • Tumor Necrosis Factor-alpha / metabolism
  • Uveitis / diagnosis
  • Uveitis / drug therapy
  • Uveitis / genetics
  • Uveitis / immunology
  • Uveitis / metabolism*
  • Uveomeningoencephalitic Syndrome / diagnosis
  • Uveomeningoencephalitic Syndrome / immunology
  • Uveomeningoencephalitic Syndrome / metabolism
  • Young Adult

Substances

  • Adrenal Cortex Hormones
  • Cytokines
  • HLA-B27 Antigen
  • IL10 protein, human
  • Interleukin-15
  • Interleukin-17
  • Tumor Necrosis Factor-alpha
  • Interleukin-10
  • Interferon-gamma
  • Cyclosporine