Due to the biological importance of nucleotides and related species, such as XNP (where X = adenosine (A), uridine (U), cytidine (C), guanosine (G), and N = mono, di, tri), FAD and NADH, the development of optical probes for these molecules has recently been an active area of research. This tutorial review focuses on the contributions between 2004-2010 concerning the fluorescent or colorimetric sensors for these biomolecules, and is organized according to their target molecule's structural classification.