Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2011 Jun;11(2):241-57.
doi: 10.1007/s10142-011-0214-7. Epub 2011 Feb 23.

Identification of Essential Genes in C. Jejuni Genome Highlights Hyper-Variable Plasticity Regions


Identification of Essential Genes in C. Jejuni Genome Highlights Hyper-Variable Plasticity Regions

Martin Stahl et al. Funct Integr Genomics. .


A microarray transposon-based tracking approach was used to identify Campylobacter jejuni genes which are required for cell growth at 37°C, under a microaerophilic atmosphere and on a rich Mueller-Hinton medium. A transposon-based mutant library, comprised of 7,201 individual mutants was constructed, representing 4.48× coverage of the genome. An analysis of genes lacking a transposon insertion revealed 195 essential gene candidates. The function of these genes represent many of the expected core functions of the cell, such as energy metabolism, macromolecule and cofactor biosynthesis, cell structural proteins as well as basic cell processes. Forty-nine hypothetical proteins were also identified, further underlining the importance of currently unknown proteins and pathways within C. jejuni. Unlike other bacteria, the essential genes were not uniformly distributed along the chromosome with three main regions lacking essential genes. These particular regions corresponded to known hyper-variable plasticity regions of C. jejuni genome indicating, as expected, that these regions are dispensable in any given C. jejuni strain. Overall, this work identified dispensable and essential genes in C. jejuni that will ultimately lead to a better understanding of Campylobacter physiology.

Similar articles

See all similar articles

Cited by 19 articles

See all "Cited by" articles


    1. PLoS One. 2010 Aug 26;5(8):e12253 - PubMed
    1. Infect Immun. 2007 Jul;75(7):3425-33 - PubMed
    1. J Clin Microbiol. 2006 Nov;44(11):4125-35 - PubMed
    1. J Bacteriol. 2004 Dec;186(23):7926-35 - PubMed
    1. Genome Res. 2009 Sep;19(9):1639-45 - PubMed

Publication types

MeSH terms


LinkOut - more resources