Sodium-dependent vitamin C transporter 2 (SVCT2) expression and activity in brain capillary endothelial cells after transient ischemia in mice

PLoS One. 2011 Feb 11;6(2):e17139. doi: 10.1371/journal.pone.0017139.

Abstract

Expression and transport activity of Sodium-dependent Vitamin C Transporter 2 (SVCT2) was shown in various tissues and organs. Vitamin C was shown to be cerebroprotective in several animal models of stroke. Data on expression, localization and transport activity of SVCT2 after cerebral ischemia, however, has been scarce so far. Thus, we studied the expression of SVCT2 after middle cerebral artery occlusion (MCAO) in mice by immunohistochemistry. We found an upregulation of SVCT2 after stroke. Co-stainings with Occludin, Von-Willebrand Factor and CD34 demonstrated localization of SVCT2 in brain capillary endothelial cells in the ischemic area after stroke. Time-course analyses of SVCT2 expression by immunohistochemistry and western blots showed upregulation in the subacute phase of 2-5 days. Radioactive uptake assays using (14)C-labelled ascorbic acid showed a significant increase of ascorbic acid uptake into the brain after stroke. Taken together, these results provide evidence for the expression and transport activity of SVCT2 in brain capillary endothelial cells after transient ischemia in mice. These results may lead to the development of novel neuroprotective strategies in stroke therapy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascorbic Acid / metabolism
  • Blood-Brain Barrier / metabolism
  • Blood-Brain Barrier / pathology
  • Brain / blood supply
  • Brain / metabolism*
  • Brain / pathology*
  • Endothelial Cells / metabolism*
  • Endothelial Cells / pathology
  • Gene Expression Regulation*
  • Infarction, Middle Cerebral Artery / metabolism
  • Infarction, Middle Cerebral Artery / pathology
  • Ischemic Attack, Transient / metabolism*
  • Ischemic Attack, Transient / pathology*
  • Male
  • Mice
  • Protein Transport
  • Sodium-Coupled Vitamin C Transporters / metabolism*
  • Time Factors
  • Up-Regulation

Substances

  • Slc23a2 protein, mouse
  • Sodium-Coupled Vitamin C Transporters
  • Ascorbic Acid